Effect of Curcumin Against Oxidation of Biomolecules by Hydroxyl Radicals
Published: October 1, 2014 | DOI: https://doi.org/10.7860/JCDR/2014/.4967
Sai Krishna Borra, Jaideep Mahendra, Prema Gurumurthy, Jayamathi, Shabeer S Iqbal, Little Mahendra
1. Research Scholar, Department of Biochemistry, Frontier Lifeline Hospital, Mogappair, Chennai, India.
2. Professor, Department of Periodontics, Meenakshi Ammal Dental College, Madhuravoyal, Chennai, India.
3. Director - Research, Meenakshi Academy of Higher Education and Research, West K.K. Nagar, Chennai, India.
4. Professor, Department of Biochemistry, Meenakshi Ammal Dental College, Madhuravoyal, Chennai, India.
5. Research Scholar, Department of Biochemistry, Frontier Lifeline Hospital, Mogappair, Chennai, India.
6. Associate Professor, Department of Periodontology, Raja Muthaiah Dental college and Hospital, Annamalai University, Chidambaram, India
Correspondence
Sai Krishna Borra,
Research Scholar, Department of Biochemistry,
Frontier Lifeline Hospital, Mogappair, Chennai - 600101, India
Phone : 07299131473, E-mail : saikrishnaborra@gmail.com
Background: Among various reactive oxygen species, hydroxyl radicals have the strongest chemical activity, which can damage a wide range of essential biomolecules such as lipids, proteins, and DNA.
Objective: The objective of this study was to investigate the beneficial effects of curcumin on prevention of oxidative damage of biomolecules by hydroxyl radicals generated in in vitro by a Fenton like reaction.
Materials and Methods: We have incubated the serum, plasma and whole blood with H2O2/Cu2+/ Ascorbic acid system for 4 hours at 37 0C and observed the oxidation of biomolecules like albumin, lipids, proteins and DNA. Results: Curcumin at the concentrations of 50,100 and 200 µmoles, prevented the formation of ischemia modified albumin, MDA, protein carbonyls, oxidized DNA and increased the total antioxidant levels and GSH significantly.
Conclusion: These observations suggest the hydroxyl radical scavenging potentials of curcumin and protective actions to prevent the oxidation of biomolecules by hydroxyl radicals.
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